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Cucumber mosaic virus

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Cucumber mosaic virus
"Cucumber mosaic virus" symptoms
Cucumber mosaic virus symptoms
Virus classification Edit this classification
(unranked): Virus
Realm: Riboviria
Kingdom: Orthornavirae
Phylum: Kitrinoviricota
Class: Alsuviricetes
Order: Martellivirales
Family: Bromoviridae
Genus: Cucumovirus
Species:
Cucumber mosaic virus
Synonyms
  • banana infectious chlorosis virus
  • coleus mosaic virus
  • cowpea banding mosaic virus
  • cowpea ringspot virus
  • cucumber virus 1
  • lily ringspot virus
  • pea top necrosis virus
  • peanut yellow mosaic virus
  • southern celery mosaic virus
  • soybean stunt virus
  • spinach blight virus
  • tomato fern leaf virus
  • pea western ringspot virus

Cucumber mosaic virus (CMV) is a plant pathogenic virus[1] in the family Bromoviridae.[2] This virus has a worldwide distribution and a very wide host range,[3] having the reputation of the widest host range of any known plant virus.[4] It can be transmitted from plant to plant both mechanically by sap and by aphids in a stylet-borne fashion. It can also be transmitted in seeds and by the parasitic weeds, Cuscuta sp. (dodder).

Hosts and symptoms

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This virus was first characterized in cucumbers (Cucumis sativus) showing mosaic symptoms in 1934,[5] hence the name Cucumber mosaic. Since then, it has been found to infect a great variety of other plants.[6] These include other vegetables such as squash, melons, peppers, eggplants, tomatoes,[7] beans,[8] carrots, celery, lettuce, spinach,[9] beets, many ornamentals and bedding plants, such as Narcissus,[10] and various weeds. Its presence has been confirmed on every continent of the world, including Antarctica.[11]

Symptoms seen with this virus include leaf mosaic or mottling (Fig. 1), yellowing, ringspots, stunting, and leaf,[12] flower and fruit distortion. CMV also induces a type of symptom on leaves known as the "shoestring" effect on many host species. This effect causes young leaves to appear narrow and the entire plant to be stunted.[13]

Specifically, CMV can cause cucumbers to turn pale and bumpy. The leaves of these plants turn mosaic and their rugosity is often changed, making leaves wrinkled and misshapen. Growth of these plants is usually also stunted and produces few flowers. Often, cucumber fruits are oddly shaped, appear gray, and tasted bitter. These appearances lead to them being referred to as "white pickles".[citation needed]

Tomato plants are usually stunted and have poorly shaped leaves, or "fernleaf", when infected by CMV. Also certain strains of CMV can cause partial or total crop loss. CMV in peppers causes slightly different symptoms than the previously mentioned. Pepper plants often have severe foliar damage, shown as mosaic and necrotic rings. Often the peppers themselves are misshapen and contain chlorotic rings and spots. In celery, CMV causes streaking and spotting and can be often confused with symptoms of the celery mosaic virus. Symptoms of CMV in lettuce, such as chlorosis, plant stunting and often do not properly head, can be very similar to those of lettuce mosaic virus.[citation needed]

CMV has also been identified in a lesser extent to a number of plant species. It is found to be a minor virus infecting Allium crops.[14][15] The virus infection causes extensive malformation on garlic plants, and mild malformation to severe necrotic streaks on onion leaves.[14][16] The severe damages on garlic plants and difficulty of aphids transmission of garlic isolate, were possible causal for the rare CMV on garlic.[17] Genetically, the two onion isolates from Turkey (CMV-14.3Po and CMV-15.5Po) are highly similar to others isolated from various plant species in Mediterranean, European, and East Asian countries.[16] CMV was also detected on American beautyberry, an important wildlife and pollinator food source plant native to North America.[18]

In plant tissue this virus makes characteristic viral inclusion bodies which can be diagnostic. They are hexagonal in shape (Fig. 2) and stain both in a protein stain and a nucleic acid stain.[19] The inclusions can also be rhomboidal, may appear hollow (Fig. 2) and can form larger aggregates. The inclusions are not uniformly distributed and can be found in epidermal (Fig. 2), mesophyll, and stomatal cells. These inclusions[19] are made up of virus particles.

Importance

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CMV is non-persistently non-circulatively transmitted by more than 80 different aphid species,[20] among other vectors. As consequences, the virus is easily spread, and can be found worldwide. CMV infects over 1200 plant species, including important crops and ornamental species. In its plant host, CMV can induce severe damage, which often lead to economical losses, as it has been proven to cause to 10-20% loss of field yield.[21] A reduction of crop yield can affect mechanisms such as photosynthesis and its ability to provide oxygen to the environment.[22]

Disease cycle

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CMV is mainly transmitted by aphids, but it can also be mechanically spread by humans in some cases. However, the mechanically spreading of this virus is not as common as the case of other virus (such as Tobacco Mosaic Virus, TMV), because CMV is not a very stable virus. When it is transmitted by aphids, this virus has an acquisition period of five to ten seconds and an inoculation period of about a minute. Nevertheless, after two minutes, the probability of inoculation largely decreases, and within two hours it is practically impossible to transmit it. Moreover, CMV can overwinter in perennial plants and weeds, as it can survive the winter in the roots of the plant and move to the aerial parts in spring, where it can be transmitted by aphids to other plants.[7]

Once the virus penetrates into the host cell, it releases its RNAs into the host cytoplasm. Then, proteins 1a and 2a are produced to enable the virus replication, which takes place in viral factories, which are subcellular compartments which increase the efficiency of this process. There, a dsRNA genome is synthesized from the ssRNA(+) and transcribed in order to obtain viral mRNAs as well as new ssRNAs. Afterwards, the capsid proteins are produced and the new viral particles are assembled. Finally, the virus is ready to move to a new cell by triggering the formation of tubular structures which mediate the movement of the virions [1]. The short-distance (cell-to-cell) movement of the virus is achieved via plasmodesmata, while the long-distance one (within the plant) occurs via the phloem.[21]

Properties

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Genome

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CMV[23] is a linear positive-sense, tripartite single-stranded RNA virus [2]. Its genome size is 8.623 kb and it is divided among RNA1 (3357 bp),[24] RNA2 (3050 bp),[25] and RNA3 (2216 bp),[26] all of which has a tRNA-like structure [3]. These three RNAs encode five proteins, proteins 1a, 2a, 2b, movement protein (MP) and coat protein (CP). While proteins 1a and 2a are responsible for the replication of the virus, protein 2b is the host-silencing suppressor.[27] The RNA is surrounded by a protein coat consisting of 32 copies of a single structural protein which form isometric particles.[28]

Virion

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This virus presents non-enveloped, icosahedral or bacilliform virions of 26-35 nm in diameter. The different RNAs are encapsidated in distinct particles, which results in a variety of virions [4].The virus is made of 180 subunits that are composed of single capsid proteins and single stranded positive RNA. The different RNAs such as satellite RNA are linked to expressing the virus.[29] Cucumber mosaic virus affects plant species where it infects them and rapidly mutates, causing difficulties in treatments attributable to its constant mutations, host range, and resistance.[30]

Environment

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CMV is naturally found in temperate areas, where aphids, one of its main vectors, are also found.[31] In addition to its presence in temperature areas, this virus can also be found in more tropical climate zones.[32] Areas such as these include California, Spain, Italy, and parts of Eastern Asia.

Diagnosis

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Plants have physical implications of being affected by this virus. A series of yellow or green pigmentation can appear predominantly in the leaves of the plant.[33] Leaf malformation, plant necrosis, plant growth inhibition, and altered fruit quality are all characteristics of plants with this virus.[32]

The presence of this virus in a plant can be confirmed serologically (ELISA), molecularly (PCR), or by host range tests.[34] These tests can be done to determine the amount of or the presence of the virus on the plants. Biological assays can also be performed to determine the specific variants of CMV affecting the plant.

Management

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This virus can negatively affect the health and sustainability of agriculture such as crops like tomatoes, peppers, and lettuce.[35] Currently there is no chemical capable of removing this virus from an infected plant, and therefore the best control is through prevention of the infection and eradication.[36] To achieve this, it is crucial to remove weeds and diseased plants from the field, as well as use clean and sanitized tools. Other options are the use of resistant varieties, or planting so-called "trap crops".[37]

References

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  1. ^ Description of Plant Viruses:What are viruses?
  2. ^ Description of Plant Viruses: Bromovirideae
  3. ^ Plant Viruses Online:Cucumber mosaic host range Archived 2008-12-15 at the Wayback Machine
  4. ^ Crop Knowledge Master: Cucumber Mosaic Virus
  5. ^ Price, W.C. 1934. Phytopathology 24: 743.
  6. ^ Douine, L., Quiot, J.B., Marchoux, G. and Archange, P. 1979. Annls. Phytopath. 11: 439
  7. ^ a b Stephen A. Ferreira; Rebecca A. Boley. "Cucumber mosaic virus - cucumber mosaic". University of Hawaii at Manoa.
  8. ^ Vegetable MD Online: Bean – Cucumber mosaic Cucumovirus
  9. ^ Vegetable MD Online: Virus Diseases of Leafy Vegetables and Celery
  10. ^ Iwaki 1972.
  11. ^ Polischuk, V.; Budzanivska, I.; Shevchenko, T.; Oliynik, S. (2007). "Evidence for plant viruses in the region of Argentina Islands, Antarctica". FEMS Microbiology Ecology. 59 (2): 409–417. Bibcode:2007FEMME..59..409P. doi:10.1111/j.1574-6941.2006.00242.x. PMID 17328120.
  12. ^ Vegetable MD Online: Important New York Vegetable Diseases
  13. ^ "Cucumber Mosaic Virus (CMV)" (PDF). AVRDC. Archived from the original (PDF) on 2006-05-26.
  14. ^ a b Štefanac, Zlata (1980-12-31). "Cucumber Mosaic Virus in Garlic". Acta Botanica Croatica. 39 (1): 21–26. ISSN 0365-0588.
  15. ^ Santosa, Adyatma Irawan; Ertunc, Filiz (August 2020). "Identification, molecular detection and phylogenetic analysis of four viruses infecting Allium cepa in Ankara Province, Turkey". Journal of Plant Diseases and Protection. 127 (4): 561–569. doi:10.1007/s41348-020-00347-5.
  16. ^ a b Santosa, Adyatma I.; Ertunc, Filiz (2021-05-13). "Characterization of two Cucumber mosaic virus isolates infecting Allium cepa in Turkey". Phytopathologia Mediterranea. 60 (1): 13–21. doi:10.36253/phyto-11840.
  17. ^ Štefanac, Zlata; Miličić, Davor (1992-12-31). "Observations on Infection of Garlic (Allium sativum L.) with Cucumber Mosaic Virus". Acta Botanica Croatica. 51 (1): 1–5. ISSN 0365-0588.
  18. ^ Stephen H. Brown; Tom Becker; Bonnie Farnsworth. "Cucumber Mosaic Virus (CMV): A Growing Problem for American Beautyberry (Callicarpa americana)" (PDF). UF/IFAS.
  19. ^ a b "Material and Methods for the Detection of Viral Inclusions / Florida Plant Viruses and Their Inclusions". Florida Department of Agriculture & Consumer Services. Archived from the original on 2014-10-13. Retrieved 2014-09-24.
  20. ^ Palukaitis, Peter; García-Arenal, Fernando (2003). "Cucumoviruses". Advances in Virus Research. 62: 241–323. doi:10.1016/s0065-3527(03)62005-1. ISBN 978-0-12-039862-1. PMID 14719367.
  21. ^ a b "Cucumber mosaic virus". Archived from the original on 2011-06-22. Retrieved 2010-10-22.
  22. ^ Song, Xing-Shun; Wang, Yan-Jie; Mao, Wei-Hua; Shi, Kai; Zhou, Yan-Hong; Nogués, Salvador; Yu, Jing-Quan (March 2009). "Effects of cucumber mosaic virus infection on electron transport and antioxidant system in chloroplasts and mitochondria of cucumber and tomato leaves". Physiologia Plantarum. 135 (3): 246–257. doi:10.1111/j.1399-3054.2008.01189.x. ISSN 0031-9317. PMID 19140890.
  23. ^ ICTVd Descriptions:Cucumber mosaic virus
  24. ^ Hayes, Robert J.; Buck, Kenneth W. (October 1990). "Complete replication of a eukaryotic virus RNA in vitro by a purified RNA-dependent RNA polymerase". Cell. 63 (2): 363–368. doi:10.1016/0092-8674(90)90169-f. PMID 2208291.
  25. ^ Bujarski, Joseph; Gallitelli, Donato; García-Arenal, Fernando; Pallás, Vicente; Palukaitis, Peter; Reddy, M. Krishna; Wang, Aiming (2019-08-01). "ICTV Virus Taxonomy Profile: Bromoviridae". Journal of General Virology. 100 (8): 1206–1207. doi:10.1099/jgv.0.001282. hdl:11586/301641. PMID 31192783.
  26. ^ Boccard, Frédéric; Baulcombe, David (April 1993). "Mutational Analysis of Cis-acting Sequences and Gene Function in RNA3 of Cucumber Mosaic Virus". Virology. 193 (2): 563–578. doi:10.1006/viro.1993.1165. PMID 8460476.
  27. ^ Kong, J.; Wei, M.; Li, G.; Lei, R.; Qiu, Y.; Wang, C.; Li, Z. H.; Zhu, S. (2018-04-06). "The cucumber mosaic virus movement protein suppresses PAMP-triggered immune responses in Arabidopsis and tobacco". Biochemical and Biophysical Research Communications. 498 (3): 395–401. doi:10.1016/j.bbrc.2018.01.072. PMID 29407169.
  28. ^ ICTVdB - Picture Gallery:Images of CMV
  29. ^ Jacquemond, Mireille (2012), "Cucumber Mosaic Virus", Viruses and Virus Diseases of Vegetables in the Mediterranean Basin, Advances in Virus Research, vol. 84, Elsevier, pp. 439–504, doi:10.1016/b978-0-12-394314-9.00013-0, ISBN 978-0-12-394314-9, PMID 22682176, retrieved 2024-04-22
  30. ^ Roossinck, Marilyn J. (March 2001). "Cucumber mosaic virus , a model for RNA virus evolution". Molecular Plant Pathology. 2 (2): 59–63. doi:10.1046/j.1364-3703.2001.00058.x. ISSN 1464-6722. PMID 20572992.
  31. ^ Jude Boucher. "Pepper IPM: Aphids and Viruses". University of Connecticut Integrated Pest Management Program. Archived from the original on 2001-02-25.
  32. ^ a b Mochizuki, Tomofumi; Ohki, Satoshi T. (April 2012). "Cucumber mosaic virus : viral genes as virulence determinants". Molecular Plant Pathology. 13 (3): 217–225. doi:10.1111/j.1364-3703.2011.00749.x. ISSN 1464-6722. PMC 6638793. PMID 21980997.
  33. ^ Dombrovsky, Aviv; Tran-Nguyen, Lucy T.T.; Jones, Roger A.C. (2017-08-04). "Cucumber green mottle mosaic virus : Rapidly Increasing Global Distribution, Etiology, Epidemiology, and Management". Annual Review of Phytopathology. 55 (1): 231–256. doi:10.1146/annurev-phyto-080516-035349. ISSN 0066-4286. PMID 28590876.
  34. ^ Khan, Shahanavaj; Jan, Arif Tasleem; Mandal, Bikash; Haq, Qazi Mohd. Rizwanul (March 2012). "Immunodiagnostics of cucumber mosaic virus using antisera developed against recombinant coat protein". Archives of Phytopathology and Plant Protection. 45 (5): 561–569. Bibcode:2012ArPPP..45..561K. doi:10.1080/03235408.2011.588043. ISSN 0323-5408.
  35. ^ Gallitelli, Donato (November 2000). "The ecology of Cucumber mosaic virus and sustainable agriculture". Virus Research. 71 (1–2): 9–21. doi:10.1016/S0168-1702(00)00184-2. PMID 11137158.
  36. ^ Pest Alert: Cucumber mosaic virus
  37. ^ Darzi, Elinor; Lachman, Oded; Smith, Elisheva; Koren, Amnon; Klein, Eyal; Pass, Nadav; Frenkel, Omer; Dombrovsky, Aviv (November 2020). "Paths of cucumber green mottle mosaic virus disease spread and disinfectant-based management". Annals of Applied Biology. 177 (3): 374–384. doi:10.1111/aab.12629. ISSN 0003-4746.

Bibliography

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Other on-line Links about CMV for growers and gardeners

  1. Texas Plant Disease Handbook: Cucumber Mosaic
  2. Weekend Gardener: Cucumber mosaic virus
  3. Ohio Floriculture:

Cucumber mosaic virus

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